منابع مشابه
Establishment of fibroblast cultures.
Fibroblasts are fairly easily isolated from a variety of tissues, in this case skin. The cells are fast growing and can be rapidly expanded from small samples. They are suitable for behavioral, functional, biochemical, and genomic studies. Skin fibroblast cultures are started from explants in which the epidermis is enzymatically removed to prevent contamination of the fibroblasts with epidermal...
متن کاملThe turnover of collagen in fibroblast cultures.
Collagen turnover was studied in mouse fibroblast cultures (3T6) by radioactive labelling and compartmental analyses. The incorporation of [C]proline into protein during continuous labelling rapidly reached a maximum value which was directly proportional to the medium specific activity. Radioactivity appeared more slowly in hydroxyproline, and gradually accumulated as cultures became enriched i...
متن کاملSpectral karyotyping of Werner syndrome fibroblast cultures.
Fibroblast cultures from two Werner syndrome patients were analyzed by spectral karyotyping. There were multiple, pseudodiploid clones in both cultures, mostly marked by random balanced reciprocal translocations. One of the cultures contained a clone with three-way exchanges involving chromosomes 2, 3, and 16. Duplication-deficiencies were exceptional, as were completely normal metaphases. The ...
متن کاملEstablishing primary adult fibroblast cultures from rodents.
The importance of using primary cells, rather than cancer cell lines, for biological studies is becoming widely recognized. Primary cells are preferred in studies of cell cycle control, apoptosis, and DNA repair, as cancer cells carry mutations in genes involved in these processes. Primary cells cannot be cultured indefinitely due to the onset of replicative senescence or aneuploidization. Henc...
متن کاملMetachromatic leukodystrophy: arylsulfatase-A deficiency in skin fibroblast cultures.
Fibroblasts cultured from the skin of a patient with metachromatic leukodystrophy have been found to manifest the biochemical defect of this inborn error of metabolism, a deficiency of arylsulfatase A. Diseased cells had less than five per cent of normal arylsulfatase-A activity, while activities of other lysosomal enzymes-including arylsulfatase B, beta-galactosidase, beta-glucuronidase, and b...
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ژورنال
عنوان ژورنال: Cell Adhesion & Migration
سال: 2007
ISSN: 1933-6918,1933-6926
DOI: 10.4161/cam.1.3.5144